Document Type : Original Article
Authors
Department of Clinical pathology, faculty of medicine, Minia University
Abstract
Highlights
Conclusion
Carbapenem resistance among pseudomonas strains require further surveillance, strong preventive measures and application of infection-control policies. In addition, to routine phenotypic methods for detecting carbapenemases production molecular confirmation by PCR of different carbapenemases producers is required for all isolates in order to identify the hidden genes. Also, periodic surveillance program is very crucial for each area to determine the most effective treatment polices.
Conflict of interest
The authors declare that there is no conflict of interest regarding the publication of this paper.
Keywords
Main Subjects
Introduction
Multidrug-resistant (MDR) P. aeruginosa is resistant to one antibiotic in three or more anti-pseudomonal antibiotics (penicillin/ cephalosporin, Carbapenems, quinolones and aminoglycosides) [3].
MDR P. aeruginosa becoming a major health concern because the organism is inherently resistant to many antibiotic classes and is able to acquire resistance to all effective antimicrobial drugs [4].
enzymes, especially IMP (imipenem active metallo-β-lactamase) and VIM (Verona integron-encoded metallo β-lactamase) [5, 6].
Carbapenemase resistance refers to Ambler classes A, B, and D [7, 8], but MβL VIM and IMP belong to Ambler B. MβLs are inhibited by ethylene-diamine-tetra-acetic acid (EDTA) and sodium mercapto-acetic acid (SMA), but not affected by β-lactase inhibitors such as clavulanic acid, sulbactam and tazobactam [9].
The aim of this study was to detect the prevalence of bla-IMP gene in P.aeruginosa strains isolated from patients at Minia university hospitals.
Material and methods
Isolation and identification P. aeruginosa
This work was conducted on 40 pseudomonas species, which isolated from clinical specimens submitted to microbiology unit at Minia University hospitals for culture and sensitivity through the period of June 2018 to September 2018. All isolates were subjected to the following:
-Routine culture on blood and MacConkey agar media for 24-48 hours incubation at 37 °C. Then identification and AST was done using (VITEK-2, bioMérieux - France).
-All Carbapenems (Imipenem and Meropenem) resistant pseudomonas isolates were preserved for evaluation of bla-IMP gene expression using PCR.
-DNA extraction was done using (Promega Co., USA) kits following the manufacturer’s protocol.
-PCR detection: specific primer designed from (bla-IMP): forward primer: (5'-CTA CCG CAG CAG AGT CTT TG-3'), Reverse primer: (5'-AAC CAG TTT TGC CTT ACC AT-3') (Operon Co., Germany) [10].
As a control for our work, the primer pair (5’-ATGGAAATGCTGAAATTCGGC-3’) and (5’-CTTCTTCAGCTCGACGCGACG-3’) was selected in order to amplify conserved regions of a target gene in P. aeruginosa and thus generate a PCR amplicon with a certain molecular weight (500 bp) that can be identified by gel electrophoresis.
Statistical analysis
Statistical Package for Social Science (Inc., Chicago, version 21). Statistical significance was recognized when p < 0.05. The numerical data were expressed as means and standard deviations. The qualitative data were expressed in frequencies and percentages. Student’s t-test was applied in order to compare between two independent groups. Frequencies of categorical variables were compared using the x2 test and Fishers’ exact test when appropriate. For quantitative data, the comparison between the three groups was performed by an analysis of variance (ANOVA) followed by post-hoc Newman-Keuls multiple comparison tests. A two tailed p < 0.05 was considered significant.
Results
In this study, 40 Pseudomonas isolates were collected from clinical specimens admitted to microbiology unit at Minia university hospitals, through the period of June 2018 to September 2018, and distributed according to the type of specimens as illustrated in table (1).
Discussion
MDR P. aeruginosa is considered a major problem; different antibiotics are commonly used for its treatment such as aminoglycosides, beta-lactamases, and quinolones [11]. The antibiotic resistance pattern in pseudomonas hesitates rapidly, especially in the case of imipenem [12]. Carbapenems (e.g. imipenem and meropenem) are often the last resort antibiotics used to treat MDR P.aeruginosa [13]. Production or acquisition of genes of MβLs (e.g. IMP and VIM) is considered the increasing cause of antibiotic resistance to Carbapenems [14, 15].
The MβLs producing P.aeruginosa in Egypt vary according to infection control policies and procedures utilized in hospitals [16]. The present study showed that 65% of clinical isolates of P. aeruginosa were resistant to Carbapenems (imipenem and meropenem). These results were near to that of Rehab Mousa et al. [17] who reported 69% of his isolates. In our study, the resistance rate of P. aeruginosa to imipenem was 62.5% and to meropenem was 65%. Carbapenem resistance rates can vary according to local antibiotic regulations, strain origin, and geographic location.
The bla-IMP gene was expressed in 30% of all pseudomonas isolates representing about 46% of carbapenem resistant strains. In Saudi Arabia, Al-Agamy et al. found that the prevalence of resistance to carbapenem was 34% and about 22% of them produced MBLs [18]. On the other hand, our results disagree with a study from Iran, which reported that only 8 (9.75%) of MBL-producing P. aeruginosa isolates were positive for bla-IMP [19]. In a study done in Iran, 100 isolates were resistant to imipenem, about 70 (70%) were found to be MBLs producers [20]. Therefore, detection of the MBL-producing strains was necessary for both; proper specific treatment and to decrease the nosocomial spread of resistance strains [21].
In the present study, it was found that P.aeruginosa isolates exhibited different rates of resistance towards aminoglycosides, which include Amikacin and Gentamicin with a percentage (60 % and 55%) respectively. Previous studies from Iran have approved the
high resistance rates of the P. aeruginosa to aminoglycosides (gentamycin, amikacin) which was similar to current results [22].
The current work revealed that the resistance towards ciprofloxacin and levofloxacin were (60% and 55%) respectively. these results disagreed with Al.Fahadawi et al who found in his study on pseudomonas isolates which gave good effectiveness towards ciprofloxacin and norfloxacin, where the percentage of sensitivity was (85.3%, 76.5%) and the percentage of resistance was (11.8%, 17.6%) respectively [23]. Whereas other researchers reported similar rates of resistance to quinolones [24]. While, Corona-Nakamura et al. [25] showed that P. aeruginosa isolates were susceptible to antibiotic ciprofloxacin. This difference may be due to the continuous development of MDR strains of P. aeruginosa worldwide.
Colistin is one of polymyxin class of antibiotics, which has a wide range of activity against most Gram-negative bacteria. Colistin nowadays is used widely for clinical application especially MDR P. aeruginosa. Based on the breakpoint reported by CLSI [26] for colistin against P. aeruginosa, a susceptible breakpoint of ≤ 2mg/L and a resistant breakpoint of ≥ 4mg/L. Interestingly, colistin was the most effective antibiotic in our study since all of our tested carbapenem resistant isolates (n=26) were found to be sensitive to colistin.
References
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