Impact of using Single File System Versus Multiple File System on Single-bacterial biofilm in Oval Canals (Comparative in- vitro study)

Abstract:

Aim of Study :

The current study aimed to assess and evaluate the effects of a single file system against a multiple file system on the eradication of single-species E. faecalis biofilm in oval canals.

Materials and Methods:

A total of 100 human Maxillary second premolars were acquired from the outpatient clinic. All collected teeth (n=100) were numbered and randomly assigned to four groups as follows: In Group I, consisting of 40 samples, instrumentation was performed utilizing a single-file method known as Reciproc Blue 40, accompanied by saline irrigation.

Group II (n=40): The samples were instrumented using the Hyflex CM multiple file system, with Saline irrigation.

Group III (n=10): A positive control group was employed to assess the survivability of bacteria during the entire experiment. In this group, the root canals were intentionally contaminated but not instrumented.

Group IV (n=10): A negative control group was included in the study to assess the sterility of the procedures. In this group, the root canals were instrumented but not contaminated. To introduce E. faecalis suspension into each root canal, 20 lL was filled through their orifice using sterile automatic micropipettes.

The bacterial colonies were enumerated, and the resulting data was quantified as colony-forming units per milliliter (C.F.U./mL).

The results indicate a statistically significant difference seen among the various groups (p<0.001). The positive control exhibited the highest value (12.39±0.16), followed by Reciproc blue (11.90±0.21) and Hyflex CM (10.95±0.36), whereas the negative control had the lowest value (0.00±0.00).