Value of flowcytometric determination of circulating endothelial progenitor cells in Behcet’s disease patients.

Mohamed, Hebatallah Ashraf; Kamal El-Din, Amel Mahmoud; Mohamed, Faten Ismail; ElSayed, Mostafa Ahmed; Mohamed, Ayat Mostafa

doi:10.21608/mjmr.2023.224689.1477

Value of flowcytometric determination of circulating endothelial progenitor cells in Behcet’s disease patients.

Document Type : Original Article

Authors

¹ clinical pathology department, faculty of medicine, minia university, minia, Egypt.

² clinical pathology faculty of medicine Minia university El minia Egypt

³ rheumatology, rehabilitation and physical medicine faculty of medicine Minia university Minia Egypt

⁴ Department of Clinical Pathology, Faculty of Medicine, Minia University, El-Minia, Egypt

⁵ clinical pathology department,faculty of medicine,Minia university

10.21608/mjmr.2023.224689.1477

Abstract

Background and aim of the study: Behcet's disease (BD) defined as a systemic vasculitis condition with multifactorial immunopathogenesis. Endothelial progenitor cells (EPCs) are essential for vascular repair and hemostasis. Our current study aimed to evaluate role of circulating EPCs in BD and correlate it with lab findings & clinical manifestations /disease activity. Methodology: The study included 30 patients (group I) suffering from BD and 25 apparently healthy subjects group(II) matched with group(I). For BD patients, clinical features and activity assessed by current disease activity form (BDCAF). For All subjects, clinical and laboratory investigations including routine tests and the determination of circulating EPCs using flowcytometry (Identified as live nucleated CD45 negative/CD34 bright/CD309positive events) were recorded. Results: There was statistically significant increase in group I regarding EPCs,TLC,AST CRP and ESR compared to group II.Circulating EPCs showed positive significant correlation with hemoglobin,AST and ALT However there were negative significant correlations with TLC, Monocytes ,Neutrophils, ESR and NLR and negative insignificant correlation with CRP and ANCA and ANCA C& P, There was negative non-significant correlation between EPCs and eye , skin and joint symptoms and BDCAF score .Conclusion:Our study demonstrated increased level of circulating EPCs in BD than controls with negative correlation with inflammatory markers and negative(nonsignificant) correlation with clinical manifestations and BDCAF score. High levels of circulating EPCs could be a good sign of endothelial healing suggesting its potential, after further studies on large cohort of patients, to serve as a maker of repair and recovery of damaged blood vessels in BD patients.

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