The Effect of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant titanium discs

Document Type : Original Article

Authors

1 Oral Medicine, Oral Diagnosis and Periodontology, Cairo University- Egypt.

2 Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Minia University.

3 Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University

4 Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Minia University

Abstract

Aim: Was to study the effect of recombinant human bone morphogenetic protein-7 (rhBMP7) on the osteoblast-like cells cultured on implant titanium discs. Methodology: The 
osteoblast-like Osteo-1 cell line was used in this experiment and derived from parietal bone 
tissue of newborn albino rats. The cells was incubated in a humid atmosphere of 95% air and 
5% carbon dioxide at 37°C. The medium was changed every 2 days. Four groups were 
conducted as follow Osteoclast like cell (Machined), Implanted Osteoclast like cell on 
Titanium (Modified), Osteoclast like cell supplemented with BMP-7 (Machined+ BMP-7) 
and Implanted Osteoclast like cell on Titanium and supplemented with BMP-7 
(Modified+BMP-7). Results: Cell proliferation was influenced by rhBMP-7, as demonstrated 
by a significant increase in collagen content after 7 and 21 days of culture (P =0.005) and a 
significant increase in alkaline phosphatase activity after 7 days (P <0.001). The addition of 
rhBMP-7 influenced alkaline phosphatase activity, and a significant increase was observed 
after 21 days (P <0.001). Conclusion: Within the limitations of the study, we concluded that 
the presence of rhBMP-7 did not influence cell adhesion to chemically modified titanium 
surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells 
cultured on this type of surface.

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