The present study aimed to estimate postmortem interval through immunohistochemical markers and histological examination of the skeletal muscle of rats at different time intervals. Fifty adult male albino rats were divided into five groups with 10 rats in each group. The rats were killed by cervical dislocation and the skeletal muscle samples were taken at times (zero, 24, 48, 72, and 96 hrs PM). The samples were subjected to histological and immunohistochemical examination. The results revealed that histological changes occurred gradually over the time since death. Immunohistochemical results of p53 and caspase-3 showed a significantly strong negative correlation with PMI. It was concluded that postmortem histological changes and immunohistochemistry of apoptotic markers (p53 and caspase-3) in skeletal muscle could be useful for estimating a postmortem interval.
Hanna, M., Abd El Wahab, S., Adel, R., & Ahmed, A. (2021). Immunohistochemical markers study from different organs of rats after death for estimation of postmortem interval: An experimental study.. Minia Journal of Medical Research, 32(2), 80-90. doi: 10.21608/mjmr.2021.231550
MLA
Morid M. Hanna; Soha A. Abd El Wahab; Rana Adel; Abeer M. Ahmed. "Immunohistochemical markers study from different organs of rats after death for estimation of postmortem interval: An experimental study.", Minia Journal of Medical Research, 32, 2, 2021, 80-90. doi: 10.21608/mjmr.2021.231550
HARVARD
Hanna, M., Abd El Wahab, S., Adel, R., Ahmed, A. (2021). 'Immunohistochemical markers study from different organs of rats after death for estimation of postmortem interval: An experimental study.', Minia Journal of Medical Research, 32(2), pp. 80-90. doi: 10.21608/mjmr.2021.231550
VANCOUVER
Hanna, M., Abd El Wahab, S., Adel, R., Ahmed, A. Immunohistochemical markers study from different organs of rats after death for estimation of postmortem interval: An experimental study.. Minia Journal of Medical Research, 2021; 32(2): 80-90. doi: 10.21608/mjmr.2021.231550
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