Effect of Amlodipine and l-Carnitin Separately and Collectively on Certain Body Parameters that Are Related to Bone Metabolism in Ovariectomized Albino Rats

Document Type : Original Article

Authors

1 Department of Physiology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt

2 Department of Physiology, Egyptian Russian University, Egypt.

3 * Department of Physiology, Egyptian Russian University, Egypt.

4 Department of Pharmacology, Faculty of Medicine, Assuit University, Egypt.

5 Department of Histology, Faculty of Medicine, Minia University, Egypt.

Abstract

Background: Amlodipine as calcium channel blocker has anti inflammatory through over 
augmentation of nitric oxide production, Anti oxidant effect by reduced superoxide radicals and 
increased the level of superoxide dismutase L-Carnitine has anti inflammatory through reacts with 
acyl group that accumulated in anoxic cells, Anti oxidant effect decreasing the superoxide anion 
production. Objective: Assessing the effect of Amlodipine and L-Carnitine Separately And 
Collectively in osteoporosis induced by ovariectomy in female albino rats. Materials and Methods: 
Fifty adult female albino rats of local strain were randomly divided into five groups of 10 animals 
each. G1: Negative control group that received no treatment G2: Postive control group 
Ovariectomized rats that will be received no treatment G3: Amlodipine treated group Ovariectomized 
rats that will be received Amlodipine (5mg/kg/day) dissolved in distilled water by intraperitoneal 
injection (after two months from ovariectomy operation ) for three weeks. G4: L-Carnitine treated 
group Ovariectomized rats that will be received L-Carnitine (100 mg/kg/day) by intra-peritoneal 
injection (after two months from ovariectomy operation ) for three weeks. G5: Amlodipine + LCarnitine Treated Group Ovariectomized 10 rats that will be received both Amlodipine at dose 
(5mg/kg/day) and L-Carnitine at a dose of 100 mg/kg/ day by intraperitoneal injection (after two 
months from ovariectomy operation) for three weeks. All rats were sacrificed and blood was 
withdrawn for biochemical examinations of Estrogen, Osteocalcine, Tumor necrosis factor α (TNFα) 
and Alkaline phosphatase (ALP). Femurs were removed for histopathological examination. Results:
The serum levels of Osteocalcin, TNFα and ALP were increased significantly in positive control group 
as compared to negative control group. Treatment with Amlodipine and L-Carnitine for three weeks 
after two months from ovareiectomy operation significantly decreased the serum levels of Osteocalcin, 
TNFα and ALP when compared to positive control group. These results suggest that amlodipine and 
L-Carnitine have protective role in osteoporosis induced by ovariectomy in female albino rats. 
 

Keywords

Main Subjects

Minia Journal of Medical Research
Volume 31, Issue 2
April 2020
Pages 228-240

Files

Share

How to cite

Statistics