Some immunological studies in cohort of systemic lupus erythematosus patients

Kamal El-Din, Amel Mahmoud; Abd El-hakeem, Mohammed Abd El-razek; Ismail, Faten; Mousa, Norhan Mohamed; Mohamed, Ayat Moustafa

doi:10.21608/mjmr.2025.392264.1977

Some immunological studies in cohort of systemic lupus erythematosus patients

Articles in Press

Document Type : Original Article

Authors

¹ clinical pathology faculty of medicine Minia university El minia Egypt

² clinical pathology, Faculty of Medicine, Minia University

³ rheumatology department minia university

⁴ Department of Clinical Pathology, Faculty of Medicine, Minia University, Minia, Egypt.

⁵ Department of Clinical Pathology, faculty of medicine, Minia University, Minia, Egypt

10.21608/mjmr.2025.392264.1977

Abstract

Background and aim of the study: Systemic lupus erythematosus (SLE) is an autoimmune disease affecting all body organs. Beside genetic, hormonal and environmental variables, unbalance between pro-inflammatory and anti-inflammatory cytokines leads to alteration of the immune system and promote tissue damage. Antinuclear antibody (ANA) and anti–double-stranded DNA (Anti-dsDNA) both play central role in both diagnosis and monitoring of SLE. This study aimed to evaluate role of ANA and Anti ds-DNA and their correlation with other laboratory markers.

Methodology: The study included ninety subjects: 40 healthy individuals as control group and 50 SLE patients. SLE patients were diagnosed according to The European League Against Rheumatism/American College of Rheumatology (EULAR/ACR) 2019 criteria for SLE. ANA and Anti ds-DNA level were assessed by EIA method.

Results: There was statistically significant increase in group I regarding ANA, Anti ds-DNA, ESR, CRP , blood culture, blood urea , A/C ratio and neutrophilic count compared to group II, ANA showed statistically significant positive correlation with CRP ,urea, creatinine, while Anti-ds DNA showed statistically significant negative correlation with neutrophilic and monocytic count.

Conclusion: This study demonstrated increased level of ANA and Anti ds-DNA in SLE patients than controls which play a significant role in early diagnosis and monitoring of SLE patients.

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